KARYTYPES OF 14 INBRED LINES AS VISUALIZED BY MULTICOLOR FLUORESCENCE IN SITU HYBRIDIZATION (FISH)

Photo By: Akio Kato

Reference: Kato, A; Lamb, JC; Birchler, J. 2004. Proc Natl Acad Sci, USA. 101:13554-13559
  Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize.

Key: Somatic chromosome karotyping of B73 and Mo17 probed with the FISH mixture described by Kato et al., 2004. Knob 180-bp repeat (blue), 5S 2-3-3 (yellow, 2L), NOR-173 clone (green, 6S), CentC (green), subtelomeric 4-12-1 clone (green), Cent4 (red, 4C), microsatellite 1-26-2 clone (red), pMTY9ER telomere-associated sequence (red), and TR-1 (white). Scale bar= 10 µm. B73 and Mo17 stocks were obtained by Jim Birchler from Ed Coe.