Zm-Ye478-REFERENCE-CAAS_FIL-1.0 genome assembly
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De Novo Genome Assembly of 12 Founder Inbred Lines

Publication: Wang, B., Hou, M., Shi, J. et al. De novo genome assembly and analyses of 12 founder inbred lines provide insights into maize heterosis. Nat Genet (2023). DOI: 10.1038/s41588-022-01283-w.

A key factor contributing to the success of hybrid maize is breeding of improved parental inbred lines and assigning them to female and male heterotic groups that allow maximum exploitation of heterosis. The three major heterotic groups, Stiff Stalk (SS), Lancaster Surecrop (Lancaster or LSC, also called Nonstiff Stalk or NSS) and Iodent (IDT), were developed in the United States. In China, both local germplasm (such as Tang SiPingTou (TSPT) and LvDa Red Cob (LRC)) along with germplasm from the US, were used to develop parental inbred lines, leading to the formation of additional heterotic groups, including the PA, TSPT, LRC, PB, X-group, and Suwan groups. 12 maize founder inbred lines (FILs) were selected as representatives of nine heterotic groups of maize (SS1, SS2, PA, X-group, Lancaster1, Lancaster2, TSPT, IDT, PB) for de novo assembly and annotation, providing a valuable genomic source for the maize research community.


Data hosted at MaizeGDB:

  • FASTA and GFF files in MaizeGDB downloads area
  • BLAST targets
  • Assembly information pages


Data overview
FIL Heterotic group Assembly Annotation Downloads Accession
A632 SS2 Zm-A632-REFERENCE-CAAS_FIL-1.0 Zm00092aa.1 link GCA_026229685.1
B73* SS1 Zm-B73-REFERENCE-GRAMENE-4.0 Zm00001d.1 link GCA_902167145.1
Chang7-2 TSPT Zm-Chang-7_2-REFERENCE-CAAS_FIL-1.0 Zm00093aa.1 link GCA_026229665.1
Dan340 Lancaster2 Zm-Dan340-REFERENCE-CAAS_FIL-1.0 Zm00094aa.1 link GCA_026229635.1
Huangzaosi TSPT Zm-Huangzaosi-REFERENCE-CAAS_FIL-1.0 Zm00095aa.1 link GCA_026228945.1
Jing724 X-group Zm-Jing724-REFERENCE-CAAS_FIL-1.0 Zm00096aa.1 link GCA_026226705.1
Jing92 TSPT Zm-Jing92-REFERENCE-CAAS_FIL-1.0 Zm00097aa.1 link GCA_026226695.1
Mo17* Lancaster1 Zm-Mo17-REFERENCE-CAU-1.0 Zm00014a.1 link GCA_003185045.1
OH43 Lancaster2 Zm-Jing724-REFERENCE-CAAS_FIL-1.0 Zm00098aa.1 link GCA_026226685.1
PH207 IDT Zm-PH207-REFERENCE-CAAS_FIL-1.0 Zm00099aa.1 link GCA_026226635.1
S37 Mixed Zm-S37-REFERENCE-CAAS_FIL-1.0 Zm00100aa.1 link GCA_026226675.1
Xu178 PB Zm-Xu178-REFERENCE-CAAS_FIL-1.0 Zm00101aa.1 link GCA_026226095.1
Ye478 PA Zm-Ye478-REFERENCE-CAAS_FIL-1.0 Zm00102aa.1 link GCA_026226105.1
Zheng58 PA Zm-Ye478-REFERENCE-CAAS_FIL-1.0 Zm00103aa.1 link GCA_026226085.1
Taken from Figure.1
*Not included in this genome assembly project

Summary of genome assemblies and annotation
Accession Assembly size (bp) Gene model count
A632 2,199,300,130 45,291
Chang7-2 2,293,228,698 42,509
Dan340 2,281,093,721 43,722
Huangzaosi 2,309,753,789 44,780
Jing724 2,220,780,054 46,320
Jing92 2,175,734,762 45,551
OH43 2,296,072,056 42,111
PH207 2,189,267,354 45,817
S37 2,304,406,946 44,720
Xu178 2,261,906,378 44,191
Ye478 2,262,426,351 44,791
Zheng58 2,246,918,942 44,845
Derived from Supplemental Figures 4 and 6.